diff --git a/gnu/packages/machine-learning.scm b/gnu/packages/machine-learning.scm
index b3df4b0d628..8d20c4f6104 100644
--- a/gnu/packages/machine-learning.scm
+++ b/gnu/packages/machine-learning.scm
@@ -1523,7 +1523,6 @@ learning.")
     (propagated-inputs (list python-numpy python-scipy))
     (native-inputs (list python-autograd
                          python-flake8-bugbear
-                         python-isort
                          python-matplotlib
                          python-pytest
                          python-pytest-cov
diff --git a/gnu/packages/mail.scm b/gnu/packages/mail.scm
index 7045361af42..831e475c85d 100644
--- a/gnu/packages/mail.scm
+++ b/gnu/packages/mail.scm
@@ -3843,7 +3843,6 @@ which sends emails to HyperKitty, the official Mailman3 web archiver.")
            python-django-rest-framework
            python-elasticsearch
            python-flufl-lock
-           python-isort
            python-lxml
            python-mailmanclient
            python-mistune
@@ -3894,7 +3893,6 @@ some configuration.")
            python-django-mailman3))
     (native-inputs
      (list python-beautifulsoup4
-           python-isort
            python-mock
            python-pdm-backend
            python-pytest
diff --git a/gnu/packages/virtualization.scm b/gnu/packages/virtualization.scm
index 03dfd08f2e0..78763b1bd5b 100644
--- a/gnu/packages/virtualization.scm
+++ b/gnu/packages/virtualization.scm
@@ -2232,7 +2232,6 @@ mainly implemented in user space.")
     (native-inputs
      (list python-avocado-framework
            python-flake8
-           python-isort
            python-setuptools
            python-setuptools-scm
            python-wheel))